Product nameAnti-6X His tag® antibody [HIS.H8] (Biotin)
See all 6X His tag® primary antibodies
DescriptionMouse monoclonal [HIS.H8] to 6X His tag® (Biotin)
Tested applicationsSuitable for: ELISA, IP, ICC/IF, WBmore details
Species reactivityReacts with: Species independent
- E. coli lysate containing a multi-epitope tagged protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab173828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
|WB||1/500 - 1/1000. Predicted molecular weight: 0.8 kDa.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
All lanes : Anti-6X His tag® antibody [HIS.H8] (Biotin) (ab173828) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 0.8 kDa
Note: Predicted MW is for the His tag alone.
ab173828 has not yet been referenced specifically in any publications.