Key features and details
- Mouse monoclonal [HIS.H8] to 6X His tag® (Biotin)
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Product nameAnti-6X His tag® antibody [HIS.H8] (Biotin)
See all 6X His tag® primary antibodies
DescriptionMouse monoclonal [HIS.H8] to 6X His tag® (Biotin)
Tested applicationsSuitable for: ELISA, IP, ICC/IF, WBmore details
Species reactivityReacts with: Species independent
- E. coli lysate containing a multi-epitope tagged protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab173828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
|WB||1/500 - 1/1000. Predicted molecular weight: 0.8 kDa.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
All lanes : Anti-6X His tag® antibody [HIS.H8] (Biotin) (ab173828) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 0.8 kDa
Note: Predicted MW is for the His tag alone.
ab173828 has not yet been referenced specifically in any publications.