Anti-6X His tag® antibody [HIS.H8] (Biotin) (ab173828)

Overview

  • Product name
    Anti-6X His tag® antibody [HIS.H8] (Biotin)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [HIS.H8] to 6X His tag® (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, IP, ICC/IF, WBmore details
  • Immunogen

    Synthetic peptide corresponding to 6X His tag® aa 1-6.
    Sequence:

    HHHHHH

  • Positive control
    • E. coli lysate containing a multi-epitope tagged protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    HIS.H8
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2000.
WB 1/500 - 1/1000. Predicted molecular weight: 0.8 kDa.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • All lanes : Anti-6X His tag® antibody [HIS.H8] (Biotin) (ab173828) at 1/1000 dilution

    Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
    Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
    Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
    Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
    Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
    Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg

    Secondary
    All lanes : Streptavidin-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 0.8 kDa



    Note: Predicted MW is for the His tag alone.

References

ab173828 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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