Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/30000.
ELISA 1/10000 - 1/100000.
ICC 1/200 - 1/500.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:
  • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). Read more (PubMed: 28604720) »
  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . Read more (PubMed: 27378283) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 17 2010

Answer

I feel the best option would be for you to bind your mutant protein to the nickel plate. The ab1269 antibody can then be used to detect the his-tag on your mutant protein. It then leaves the HRP on the antibody free for detection. If you coat the plate with the ab1269 antibody, it will bind your mutant protein, but without a secondary antibody, you may have no means of detecting this.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Recombinant protein
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: PBS 10% FC
Type
Direct

Dr. Michael Aigner

Verified customer

Submitted Aug 15 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Sunil Verma

Verified customer

Submitted Aug 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up