Overview

  • Product name

    Anti-6X His tag® antibody (HRP)
    See all 6X His tag® primary antibodies
  • Description

    Rabbit polyclonal to 6X His tag® (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    ab197049 recognizes His-tag proteins.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to 6X His tag® conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    HHHHHH

  • Positive control

    • His-tag fusion protein; His-tag protein expressed cell lysate and enriched His-tag protein by IP.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 50% Glycerol, 50% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • All lanes : Anti-6X His tag® antibody (HRP) (ab197049) at 1 µg/ml

    Lane 1 : His-tag fusion protein at 5 µg
    Lane 2 : His-tag fusion protein at 20 µg

    Predicted band size: 50 kDa

  • All lanes : Anti-6X His tag® antibody (HRP) (ab197049) at 1 µg/ml

    Lane 1 : His-tag protein in lysate (original cell lysate)
    Lane 2 : His-tag protein in lysate (after IP)
    Lane 3 : His-tag protein in 100 µL of the IP eluting buffer, enriched with 25 µg of the anti-His tag from 2 mL of the original lysate

    Lysates/proteins at 10 µl per lane.

    Predicted band size: 50 kDa

References

This product has been referenced in:

  • Tian P  et al. Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac. BMC Cancer 18:1286 (2018). Read more (PubMed: 30577849) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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