Overview

  • Product name

    Anti-6X His tag® antibody (HRP)
    See all 6X His tag® primary antibodies
  • Description

    Chicken polyclonal to 6X His tag® (HRP)
  • Host species

    Chicken
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Myeloma

    unknown
  • Isotype

    IgY
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. 1/1000.
ELISA Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:

  • Burnside D  et al. In Silico Engineering of Synthetic Binding Proteins from Random Amino Acid Sequences. iScience 11:375-387 (2019). Read more (PubMed: 30660105) »
  • Dragovic SM  et al. Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. Cell Host Microbe 23:523-535.e5 (2018). Read more (PubMed: 29649443) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Recombinant protein (protein produced in mammalian expression system)
Loading amount
1 µg
Specification
protein produced in mammalian expression system
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 29 2007

Question
Answer

Thank you for your enquiry. Yes, this product will detect N-terminal HIS tags.

Read More

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