Product nameAnti-6X His tag® antibody [RM146]
See all 6X His tag® primary antibodies
DescriptionRabbit monoclonal [RM146] to 6X His tag®
Specificityab200537 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, IPmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to 6X His tag®. Mixture of a peptide with 6X His tag® at the N-terminus and another peptide with 6X His tag® at the C-terminus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab200537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.5 - 2 µg/ml.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 0.1 - 1 µg/ml.|
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml.|
|IP||Use a concentration of 0.5 - 2 µg/ml.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
All lanes : Anti-6X His tag® antibody [RM146] (ab200537) at 0.2 µg/ml
Lane 1 : Untransfected 293T cells
Lane 2 : Transfected with His-Tag fusion protein X 293T cells
Lane 3 : E. coli lysate without His-Tag Protein Y
Lane 4 : E. coli lysate with His-Tag Protein Y
The diluent was 5% milk/TBST.
ab200537 at 0.5 - 2 µg/mL immunoprecipitating 6X His tag® in 293T whole cell lysate.
Lane 1 (input): 293T whole lysate control
Lane 2 (+): Rabbit monoclonal IgG instead of ab200537 in 293T whole cell lysate
Lane 3 (-): ab200537 + 293T whole cell lysate
Immunohistochemical analysis of 293T cells expressing His-Tag nuclear protein X with ab200537 at 0.1 - 1 µg/mL. The negative control in the inset shows immunohistochemical staining of untransfected HepG2 cells with ab200537 at 0.1 - 1 µg/mL.
ab200537 has not yet been referenced specifically in any publications.