Recombinant

Recombinant Anti-6X His tag® antibody [RM146] (ab200537)

Overview

  • Product name

    Anti-6X His tag® antibody [RM146]
    See all 6X His tag® primary antibodies
  • Description

    Rabbit monoclonal [RM146] to 6X His tag®
  • Host species

    Rabbit
  • Specificity

    ab200537 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to 6X His tag®. Mixture of a peptide with 6X His tag® at the N-terminus and another peptide with 6X His tag® at the C-terminus.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM146
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.5 - 2 µg/ml.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
IP Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • All lanes : Anti-6X His tag® antibody [RM146] (ab200537) at 0.2 µg/ml

    Lane 1 : Untransfected 293T cells
    Lane 2 : Transfected with His-Tag fusion protein X 293T cells
    Lane 3 : E. coli lysate without His-Tag Protein Y
    Lane 4 : E. coli lysate with His-Tag Protein Y


    The diluent was 5% milk/TBST.

  • ab200537 at 0.5 - 2 µg/mL immunoprecipitating 6X His tag® in 293T whole cell lysate.

    Lane 1 (input): 293T whole lysate control

    Lane 2 (+): Rabbit monoclonal IgG instead of ab200537 in 293T whole cell lysate

    Lane 3 (-): ab200537 + 293T whole cell lysate

  • Immunohistochemical analysis of 293T cells expressing His-Tag nuclear protein X with ab200537 at 0.1 - 1 µg/mL. The negative control in the inset shows immunohistochemical staining of untransfected HepG2 cells with ab200537 at 0.1 - 1 µg/mL. 

References

ab200537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200537.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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