Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM146] to 6X His tag®
- Suitable for: ICC, Flow Cyt, WB, IP
- Reacts with: Species independent
Product nameAnti-6X His tag® antibody [RM146]
See all 6X His tag® primary antibodies
DescriptionRabbit monoclonal [RM146] to 6X His tag®
ab200537 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Tested applicationsSuitable for: ICC, Flow Cyt, WB, IPmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to 6X His tag®. Mixture of a peptide with 6X His tag® at the N-terminus and another peptide with 6X His tag® at the C-terminus.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab200537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 0.5 - 2 µg/ml.|
|Flow Cyt||Use a concentration of 0.5 - 2 µg/ml.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 0.1 - 1 µg/ml.|
|IP||Use a concentration of 0.5 - 2 µg/ml.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
All lanes : Anti-6X His tag® antibody [RM146] (ab200537) at 0.2 µg/ml
Lane 1 : Untransfected 293T cells
Lane 2 : Transfected with His-Tag fusion protein X 293T cells
Lane 3 : E. coli lysate without His-Tag Protein Y
Lane 4 : E. coli lysate with His-Tag Protein Y
The diluent was 5% milk/TBST.
ab200537 at 0.5 - 2 µg/mL immunoprecipitating 6X His tag® in 293T whole cell lysate.
Lane 1 (input): 293T whole lysate control
Lane 2 (+): Rabbit monoclonal IgG instead of ab200537 in 293T whole cell lysate
Lane 3 (-): ab200537 + 293T whole cell lysate
Immunohistochemical analysis of 293T cells expressing His-Tag nuclear protein X with ab200537 at 0.1 - 1 µg/mL. The negative control in the inset shows immunohistochemical staining of untransfected HepG2 cells with ab200537 at 0.1 - 1 µg/mL.
ab200537 has not yet been referenced specifically in any publications.