Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RMH01] to 6X His tag®
- Suitable for: ELISA, Sandwich ELISA, WB
- Reacts with: Species independent
Product nameAnti-6X His tag® antibody [RMH01]
See all 6X His tag® primary antibodies
DescriptionHuman monoclonal [RMH01] to 6X His tag®
ab219465 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
Species reactivityReacts with: Species independent
Synthetic peptide (His-tag). (Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus).
- WB: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (K36M) protein; Sandwich ELISA: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein; purified HisTag-PCSK9 recombinant protein
ab219465 is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (ab200537), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab219465 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab219465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Sandwich ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.2 µg/ml.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50µL/well of different concentrations of ab219465 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of ab219465 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody
All lanes : Anti-6X His tag® antibody [RMH01] (ab219465) at 0.2 µg/ml
Lane 1 : 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Lane 2 : 293T cells untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
All lanes : HRP conjugated anti-Human IgG secondary antibody
ab219465 has been referenced in 1 publication.
- Luo S et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). PubMed: 29725470