Overview

  • Product name

    Anti-6X His tag® antibody [RMH01]
    See all 6X His tag® primary antibodies
  • Description

    Human monoclonal [RMH01] to 6X His tag®
  • Host species

    Human
  • Specificity

    ab219465 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
  • Tested applications

    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide (His-tag). (Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus).

  • Positive control

    • WB: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (K36M) protein; Sandwich ELISA: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein; purified HisTag-PCSK9 recombinant protein
  • General notes

    ab219465 is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (ab200537), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab219465 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RMH01
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab219465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.2 µg/ml.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50µL/well of different concentrations of ab219465 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.

  • A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of ab219465 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody

  • All lanes : Anti-6X His tag® antibody [RMH01] (ab219465) at 0.2 µg/ml

    Lane 1 : 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
    Lane 2 : 293T cells untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein

    Secondary
    All lanes : HRP conjugated anti-Human IgG secondary antibody

References

This product has been referenced in:

  • Luo S  et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). Read more (PubMed: 29725470) »
See 1 Publication for this product

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