• Nature

Associated products


Our Abpromise guarantee covers the use of ab14943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-6X His tag® antibody (ab14923)

  • Form
  • Additional notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • 6 His epitope tag
    • Hexa His tag
    • HHHHHH epitope tag
    • HHHHHH tag
    • His tag
    • Polyhistidine Tag
    see all
  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.


This product has been referenced in:
  • Chen J  et al. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog 11:e1005053 (2015). Read more (PubMed: 26226614) »
  • Rani N  et al. FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis. Nat Commun 3:749 (2012). Read more (PubMed: 22434192) »
See all 2 Publications for this product

Customer reviews and Q&As


Ab14941 should have N terminal cys and ab14943 should have a C terminal cys.

Our peptides are supplied as lyophilised powder and re-suspended in TK buffer so contain the following:
30 mM HEPES,
150 mM NaCl,
2 mM EDTA,
0.001% tween 20,
pH 6.75

The EDTA in the buffer will chelate metal ions

Read More

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