Product name7-AAD Staining Solution
Sample typeSuspension cells
7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml). 7-AAD can be used in place of propidium iodide (PI) for the exclusion of non-viable cells in flow cytometry analysis. This product can be used in combination with PE (phycoerythrin), and FITC conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is the minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Exitation = 540 nm / emission = 645 nm.
Tested applicationsSuitable for: Flow Cytmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 400 tests 7-AAD Staining Solution 1 x 2ml
Our Abpromise guarantee covers the use of ab228563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 5µl for 106 cells.
Incubate for 5 minutes at room temperature before analysis.
ab228563 has not yet been referenced specifically in any publications.