Overview

  • Product name
    7-AAD Staining Solution
  • Sample type
    Suspension cells
  • Assay type
    Cell-based
  • Product overview

    7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml). 7-AAD can be used in place of propidium iodide (PI) for the exclusion of non-viable cells in flow cytometry analysis. This product can be used in combination with PE (phycoerythrin), and FITC conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is the minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).


    Exitation = 540 nm / emission = 645 nm.

  • Tested applications
    Suitable for: Flow Cytmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 400 tests
    7-AAD Staining Solution 1 x 2ml

Applications

Our Abpromise guarantee covers the use of ab228563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

Incubate for 5 minutes at room temperature before analysis.

Protocols

References

ab228563 has not yet been referenced specifically in any publications.

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