Product name7-(Diethylamino)coumarin-3-carbohydrazide, derivatizing agent for carboxylic acid detection
DescriptionDerivatizing agent for carboxylic acid detection.
Derivatizing agent for detection of carboxylic acid and carbonylated molecules such as lipid peroxidation products (LPP). Has also been used for fluorescence detection of monoterpene alcohols.
Fluorescence: λex 450 nm; λem 468 nm in methanol.
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Milic I & Fedorova M Derivatization and detection of small aliphatic and lipid-bound carbonylated lipid peroxidation products by ESI-MS. Methods Mol Biol 1208:3-20 (2015). Read more (PubMed: 25323495) »
- Ni Z et al. Identification of carbonylated lipids from different phospholipid classes by shotgun and LC-MS lipidomics. Anal Bioanal Chem 407:5161-73 (2015). Read more (PubMed: 25701423) »
- Milic I et al. Simultaneous detection of low and high molecular weight carbonylated compounds derived from lipid peroxidation by electrospray ionization-tandem mass spectrometry. Anal Chem 85:156-62 (2013). Read more (PubMed: 23186270) »
- Meesters RJ et al. Sensitive determination of monoterpene alcohols in urine by HPLC-FLD combined with ESI-MS detection after online-solid phase extraction of the monoterpene-coumarincarbamate derivates. J Chromatogr B Analyt Technol Biomed Life Sci 875:444-50 (2008). Read more (PubMed: 18845490) »
- Touchstone JC & Fang X Fluorometric labeling of tetrahydroprogesterones. Steroids 56:601-5 (1991). Read more (PubMed: 1819872) »