Product name7-Ethoxy-4-trifluoromethylcoumarin, Fluorescent Cytochrome P450 substrate
DescriptionFluorescent Cytochrome P450 substrate.
- Ethyl 4-(trifluoromethyl)umbelliferyl ether
Fluorescent substrate for monitoring Cytochrome P450 activity.
Fluorescence: λex 333 nm; λem 415 nm in methanol.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Jang HH et al. Functional importance of a peripheral pocket in mammalian cytochrome P450 2B enzymes. Arch Biochem Biophys 584:61-9 (2015). Read more (PubMed: 26319176) »
- Jang HH et al. The role of cytochrome P450 2B6 and 2B4 substrate access channel residues predicted based on crystal structures of the amlodipine complexes. Arch Biochem Biophys 545:100-7 (2014). Read more (PubMed: 24445070) »
- Buters JT et al. A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarin. Biochem Pharmacol 46:1577-84 (1993). Read more (PubMed: 8240414) »