7-Ethoxy-4-trifluoromethylcoumarin, Fluorescent Cytochrome P450 substrate (ab145347)

Overview

  • Product name

    7-Ethoxy-4-trifluoromethylcoumarin, Fluorescent Cytochrome P450 substrate
  • Description

    Fluorescent Cytochrome P450 substrate.
  • Alternative names

    • 7-EFC
    • 7-Ethoxy-4-(trifluoromethyl)coumarin
    • EFC
    • Ethyl 4-(trifluoromethyl)umbelliferyl ether
  • Biological description

    Fluorescent substrate for monitoring Cytochrome P450 activity.

  • Purity

    > 98%
  • General notes

    Fluorescence: λex 333 nm; λem 415 nm in methanol.

  • CAS Number

    115453-82-2
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    333nm
  • Emission

    415nm
  • Molecular weight

    258.19
  • Molecular formula

    C12H9F3O3
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Jang HH  et al. Functional importance of a peripheral pocket in mammalian cytochrome P450 2B enzymes. Arch Biochem Biophys 584:61-9 (2015). Read more (PubMed: 26319176) »
  • Jang HH  et al. The role of cytochrome P450 2B6 and 2B4 substrate access channel residues predicted based on crystal structures of the amlodipine complexes. Arch Biochem Biophys 545:100-7 (2014). Read more (PubMed: 24445070) »
  • Buters JT  et al. A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarin. Biochem Pharmacol 46:1577-84 (1993). Read more (PubMed: 8240414) »

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