7-Methoxy-4-(trifluoromethyl)coumarin, Fluorescent substrate for oxidoreductases (ab145426)

Overview

  • Product name

    7-Methoxy-4-(trifluoromethyl)coumarin, Fluorescent substrate for oxidoreductases
  • Description

    Fluorescent substrate for oxidoreductases, especially for Cytochrome P450.
  • Alternative names

    • 7-methoxy-4-(trifluoromethyl)-coumarin
    • 7-methoxy-4-trifluoromethylcoumarin
    • 7-MFC
    • Methyl 4-(trifluoromethyl)umbelliferyl ether
    • MFC
    see all
  • Biological description

    Fluorescent substrate for oxidoreductases, especially for Cytochrome P450.

  • Purity

    > 99%
  • General notes

    Fluorescence: λex 333 nm; λem 416 nm in methanol

  • CAS Number

    575-04-2
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    333nm
  • Emission

    416nm
  • Chemical name

    Methyl 4-(trifluoromethyl)umbelliferyl ether
  • Molecular weight

    244.17
  • Molecular formula

    C11H7F3O3
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO and in ethanol
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Wilderman PR  et al. Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme. FEBS J 279:1607-20 (2012). Read more (PubMed: 22051155) »
  • Makaji E  et al. Effects of cytochrome P450 inhibitors on the biotransformation of fluorogenic substrates by adult male rat liver microsomes and cDNA-expressed rat cytochrome P450 isoforms. Toxicol Sci 113:293-304 (2010). Read more (PubMed: 19858067) »
  • Oezguen N  et al. Identification and analysis of conserved sequence motifs in cytochrome P450 family 2. Functional and structural role of a motif 187RFDYKD192 in CYP2B enzymes. J Biol Chem 283:21808-16 (2008). Read more (PubMed: 18495666) »

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