Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

Mouse monoclonal 8-Hydroxy-2'-deoxyguanosine antibody [N45.1]. Validated in IHC, ICC/IF. Cited in 35 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]
  • Description

    Mouse monoclonal [N45.1] to 8-Hydroxy-2'-deoxyguanosine
  • Host species

    Mouse
  • Specificity

    Nineteen analogues of 8-Hydroxy-2'-deoxyguanosine {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%).
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin

  • Positive control

    • Epidermis from hairless mouse treated for 4 weeks with chronic UVB irradiation, or kidney treated with Fe-NTA.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 27th February 2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab48508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

For mouse on mouse staining we recommend uisng MOM kit - ab127055.

Target

  • Relevance

    8-hydroxy-2'-deoxyguanosine is a biomarker of oxidative DNA damage.
  • Alternative names

    • 8 OHdG antibody
    • 8-OHdG antibody
    • 8OHdG antibody

Images

  • De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-Hydroxy-2'-deoxyguanosine (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.

    Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND: chow diet group, HFD: High fat diet, HFD-QH: High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.

  • ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • Ab48508, at a concentration of 5 µg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.
  • ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human spermatozoa by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using triton in 4% BSA for 15 minutes at room temperature, blocked with 4% BSA for 30 minutes at 20°C and then incubated using ab48508 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 350 conjugated goat anti-mouse monoclonal, used at a 1/250 dilution.

    Staining from left to right: 1. TUNEL 2. ab48508 3. DIC 4. Merge.

    See Abreview

References

This product has been referenced in:

  • Jianrong S  et al. DUSP14 rescues cerebral ischemia/reperfusion (IR) injury by reducing inflammation and apoptosis via the activation of Nrf-2. Biochem Biophys Res Commun 509:713-721 (2019). Read more (PubMed: 30638656) »
  • Whongsiri P  et al. Oxidative stress and LINE-1 reactivation in bladder cancer are epigenetically linked through active chromatin formation. Free Radic Biol Med 134:419-428 (2019). Read more (PubMed: 30703483) »
See all 44 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PH=6 ,citrate buffer
Permeabilization
No
Specification
skin
Blocking step
Rodent Block from ab127055 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% Formalin

Abcam user community

Verified customer

Submitted Nov 10 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - 0.3%triton-X
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Yoshihisa Koyama

Verified customer

Submitted Dec 22 2017

Answer

Thank you for your enquiry regarding ab48508.

Would you be so kind to confirm if the tube has been centrifuged before opening the lid? It would help me if you could specify how much volume of the liquid was in the vial?

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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Answer

Thank you for contacting us.

I can recommend the antibody ab48508 against 8-hydroxy-2'-deoxyguanosine. This antibody has been tested in immunohistochemistry on paraffin embedded tissue sections (IHC-P).
https://www.abcam.com/index.html?datasheet=48508 (or use the following: https://www.abcam.com/index.html?datasheet=48508).

Alternatively, we do have also the ab264842 which has been tested in IHC-P.
https://www.abcam.com/index.html?datasheet=26842 (or use the following: https://www.abcam.com/index.html?datasheet=26842).

All these antibodies will also work on mouse tissue. As they are however mouse antibodies, you must be careful not to introduce background by the detection with the secondary antibody. To avoid such a problem, I can recommend you to block endogenous mouse immunoglobulins on the tissue slides. This can be done with our MOM kit for example (ab127055). https://www.abcam.com/index.html?datasheet=127055 (or use the following: https://www.abcam.com/index.html?datasheet=127055).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

I am writing to you as I have been informed by our logistic group that ourAnti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1], AB48508, now ships with a concentration of 1.0mg/ml instead of 0.1mg/ml. As such the aliquot you receive will contain 50ug in a 50ul volume. Please let me know if this is alright to you and I will have the order released immediately.

This product is covered by our Abpromise guarantee. Should you experience any problems with this product we will replace or refund it under our Abpromise guarantee, if it is being used according to specifications listed on our datasheet.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not parrive with the volume as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for your enquiry.

Both these antibodies are designed to detect a specific DNA modification which is a species-independent reactivity. So yes, both antibodies are suitable for use in rat.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer

I have heard back about ab48508.
The antibody ab48508 (anti 8-OHdG monoclonal antibody) is specific for
8-Hydroxy-2'-deoxyguanosine(8-OHdG). 8-Hydroxyguanosine (8OHG) demonstrate
minimal crossreactivity (less than 1%).
I will get back to you as soon as I hear back about the other antibody.
Once again thank you for your patience.

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Answer

Thank you for your reply.
I have still not heard back from all of those products. I am going to send email reminders and follw up with what is happening. I will get back to you as soon as I find out anything.
Thank you for your patience.

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Answer

Thank you for getting back to me and for confirming the order details. It is very much appreciated.



This is to let you know that I have placed a new order for you - for one vial ofab48508 as a free of charge replacement (exchange for the original item ab26842) and the new order number is 1049930.



I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

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1-10 of 17 Abreviews or Q&A

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