Overview

  • Product name
    8-hydroxy 2 deoxyguanosine ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Spiked 90 < 5%
    Inter-assay
    Sample n Mean SD CV%
    Spiked 90 < 5%
  • Sample type
    Urine, Plasma, Cell Lysate
  • Assay type
    Competitive
  • Sensitivity
    = 0.59 ng/ml
  • Range
    0.94 ng/ml - 60 ng/ml
  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Product overview

    8-hydroxy 2 deoxyguanosine ELISA Kit (8-OHdG) (ab201734) is a competitive assay that can be used for the quantification of 8-OHdG in urine, cell culture, plasma, and other sample matrices. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 0.94 - 60 ng/mL, with a sensitivity of 0.59 ng/mL. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.


    It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results.


     

  • Notes

    8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.

    In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7- 13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/mL as determined by LC-MS.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    8-hydroxy-2-deoxy Guanosine Antibody Diluent (Blue) 1 x 13ml
    8-Hydroxy-2-Deoxy Guanosine BSA Coated Plate 1 unit
    8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody 1 x 75µl
    8-hydroxy-2-deoxy Guanosine Sample and Standard Diluent (Red) 1 x 50ml
    8-hydroxy-2-deoxy Guanosine Standard 1 x 100µl
    8-hydroxy-2-deoxy Guanosine Stop Solution 1 x 13ml
    8-hydroxy-2-deoxy Guanosine TMB Substrate 1 x 13ml
    8-hydroxy-2-deoxy Guanosine Wash Buffer Concentrate (10X) 1 x 50ml
    Plate Cover 2 units
  • Research areas
  • Relevance
    Oxidative damage of DNA or RNA by reactive oxygen and nitrogen species produces 8-hydroxy Guanine. This can serve as a measure of oxidative stress.
  • Alternative names
    • 8 OH dG

Applications

Our Abpromise guarantee covers the use of ab201734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • 8-hydroxy 2 deoxyguanosine measured in urine (dilution test range: 1/10-1/200; duplicates; +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Typical standard curve

  • Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Schematic

  • Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Recovery of 8-hydroxy-2-deoxy Guanosine from urine

  • Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Oxidation of Guanosine

Protocols

References

This product has been referenced in:
  • Antunes-Lopes T  et al. The Impact of Chronic Pelvic Ischemia on LUTS and Urinary Levels of Neuroinflammatory, Inflammatory, and Oxidative Stress Markers in Elderly Men: A Case-control Study. Urology 123:230-234 (2019). Read more (PubMed: 30219559) »
  • Devos D  et al. A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis. Sci Rep 9:2918 (2019). Read more (PubMed: 30814647) »
See all 19 Publications for this product

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