Overview

  • Product name

    8-iso-PGF2 alpha ELISA Kit
    See all 8-iso-PGF2a kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 12 232pg/ml 11%
    Medium 12 1128pg/ml 4.4%
    High 12 8263pg/ml 5.8%
    Inter-assay
    Sample n Mean SD CV%
    Low 8 183pg/ml 11%
    Medium 8 855pg/ml 8.8%
    High 8 6239pg/ml 5%
  • Sample type

    Cell culture supernatant, Urine, Tissue
  • Assay type

    Competitive
  • Sensitivity

    16.3 pg/ml
  • Range

    6.1 pg/ml - 100000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 101.58 % - %
    Tissue Culture Media 129.9 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s 8-iso-PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF2 alpha in tissue culture media and urine.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2 alpha antigen and a polyclonal rabbit antibody specific to 8-iso-PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2 alpha captured in the plate.

  • Notes

    The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F in biological fluids such as urine and in culture supernatants. 8-iso-PGF is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF has been shown to circulate in plasma and is excreted in urine.

     

    CompoundCross Reactivity
     8-iso-PGF100%
     PGF4.6%
     PGF1.85%
     PGE10.19%
     TXB20.023%
     PGB10.02%
     PGE30.012%
     6-keto-PGF0.008%
     13, 14-dihydro-15-keto-PGF0.005%
     6,15-keto-13, 14-dihydro-PGF<0.001%
     8-iso-PGE1<0.001%
     PGA2<0.001%
     PGJ2<0.001%
     2-Arachidonoylglycerol<0.001%
     Anandamide<0.001%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133025

Protocols

References

ab133025 has not yet been referenced specifically in any publications.

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