Overview

  • Product name

    8-iso-PGF2alpha ELISA Kit
    See all 8-iso-PGF2a kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    970 pg/mL 16 11.3%
    3264 pg/mL 16 5.7%
    5092 pg/mL 16 5.1%
    Inter-assay
    Sample n Mean SD CV%
    1473 pg/mL 8 5.4%
    4501 pg/mL 8 5.8%
    6679 pg/mL 8 10.4%
  • Sample type

    Serum, Plasma, Tissue
  • Assay type

    Competitive
  • Sensitivity

    40 pg/ml
  • Range

    160 pg/ml - 100000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93.8 % - %
    Heparin Plasma 97.2 % - %
    EDTA Plasma 109.8 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s Direct 8-iso-PGF2alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF in Biological fluids.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF2alpha antigen and a rabbit polyclonal antibody specific to 8-iso-PGF2alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF2alpha captured in the plate.

  • Notes

    The 8-epimer of Prostaglandin F (8-iso-PGF) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis and a mutagen in 3T3 cells and in vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator and is able to modify the fluidity and integrity of membranes. 8-isoPGF has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF typically require the alkaline hydrolysis of 8-iso-PGF esters from tissues, followed by length procedures involving extractions, phase separations and thin layer chromatography.

    Cross Reactivity

    Compound% Cross Reactivity
    8-iso-PGF100
    PGF4.6
    PGF1.85
    PGE10.19
    TXB20.023
    PGB10.02
    PGE30.012
    6-keto-PGF0.008
    13,14-dihydro-15-keto-PGF0.008
    6,15-keto-13,14-dihydro-PGF0.005
    8-iso-PGE1<0.001
    PGA2<0.001
    PGJ2<0.001
    2-Arachidonoylglycerol<0.001
    Anandamide<0.001
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133043. 2 Hour Format.

  • Representative Standard Curve using ab133043. Overnight Format.

Protocols

References

ab133043 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer


For ab133043 I received the following information:

Samples should be stored frozen at -20C or lower, prior to analysis. They subsequently would need to be powdered prior to hydrolysis as well. Kidney and glomerula tissue has not been specifically tested.
Assuming there is enough marker to detect and the customer can demonstrate dilutional linearity then this assay could work well for them for their tissue of interest.

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