Overview

  • Product name

    8 isoprostane ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 12 0.43%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12 1.75%
  • Sample type

    Urine, Serum, Plasma, Cell culture extracts, Tissue
  • Assay type

    Competitive
  • Sensitivity

    1 pg/ml
  • Range

    0.005 ng/ml - 5 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s 8 isoprostane competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 8 isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissues phospholipids by oxygen radicals. A recent NIH-sponsored study on Biomarkers of Oxidative Stress has indicated that 8 isoprostane is the best index of oxidative injury in a well-accepted oxidant stress rat model. In addition, plasma 8 isoprostane levels were found to be elevated in elderly subjects with severe hypertension and in urine from subjects with high fat diet-induced liver steatosis.

    This kit can be used for the determination of 8 isoprostane in diluted urine, serum, plasma, cells and tissues following proper isolation and purification of the eicosanoid from the isoprostane-containing sample.

     

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab175819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Example of standard curve obtained with ab175819.

Protocols

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

8-Isoprostane

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Works good and used for Human Urine samples.

Abcam user community

Verified customer

Submitted Sep 28 2018

Answer

Thank you for contacting us.





The best places to stop are after drying the ethyl acetate layer. The sample can be stored at minus twenty degrees for an extensive period of time.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer



This kit can be used with any species as the isoprostane is a well conserved compound throughout species.

The 96-well plate is coated with anti 8-isoprostane antibody in each well, which will capture the samples 8 isoprostane to be measured.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up