Anti-A1CF/ACF antibody (ab89050)
Key features and details
- Mouse polyclonal to A1CF/ACF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-A1CF/ACF antibody
See all A1CF/ACF primary antibodies -
Description
Mouse polyclonal to A1CF/ACF -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human A1CF/ACF aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_620311.1 -
Positive control
- Placenta or HeLa whole cell lysate (ab150035).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. |
Target
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Function
Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. -
Tissue specificity
Widely expressed with highest levels in brain, liver, pancreas, colon and spleen. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Domain
The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity. -
Cellular localization
Nucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1. - Information by UniProt
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Database links
- Entrez Gene: 29974 Human
- SwissProt: Q9NQ94 Human
- Unigene: 282795 Human
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Alternative names
- A1CF antibody
- A1CF_HUMAN antibody
- ACF antibody
see all
Images
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Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + Human placenta lysate at 50 µg
Predicted band size: 65 kDa -
Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 65 kDa -
All lanes : Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml
Lane 1 : A1CF/ACF-transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Datasheets and documents
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Datasheet download
References (2)
ab89050 has been referenced in 2 publications.
- Liu Q et al. High APOBEC1 Complementation Factor Expression Positively Modulates the Proliferation, Invasion, and Migration of Endometrial Cancer Cells Through Regulating P53/P21 Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32818382
- Fossat N et al. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO Rep : (2014). PubMed: 24916387