Anti-ABCA1 antibody (ab7360)
Key features and details
- Rabbit polyclonal to ABCA1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ABCA1 antibody
See all ABCA1 primary antibodies -
Description
Rabbit polyclonal to ABCA1 -
Host species
Rabbit -
Specificity
ab7360 has previously been shown to work well in ICC/IF on HeLa cells. However recent batches of this antibody, do not work in this application. We would recommend ab18180 for researchers wanting to detect ABCA1 in ICC/IF. For further information, please contact our Scientific Support Team. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human ABCA1. Synthetic peptide: The immunogen is generated from within residues 1100-1300 of Human ABCA1
Database link: O95477 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
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IHC-P |
1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel. |
IHC-P
1/500. |
Target
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Function
cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. -
Tissue specificity
Widely expressed, but most abundant in macrophages. -
Involvement in disease
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
Domain
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
Post-translational
modificationsPhosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 19 Human
- Entrez Gene: 11303 Mouse
- Omim: 600046 Human
- SwissProt: O95477 Human
- SwissProt: P41233 Mouse
- Unigene: 429294 Human
- Unigene: 277376 Mouse
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Alternative names
- ABC 1 antibody
- ABC Transporter 1 antibody
- ABC-1 antibody
see all
Images
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All lanes : Anti-ABCA1 antibody (ab7360)
Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
Lane 2 : RAW 264.7 whole cell lysate untreated
Lysates/proteins at 25 µg per lane.
Predicted band size: 220 kDa -
ab7360, staining ABCA1 in human prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.
The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separ -
Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate
Datasheets and documents
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SDS download
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Datasheet download
References (59)
ab7360 has been referenced in 59 publications.
- Lu Q et al. Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticity. iScience 25:104201 (2022). PubMed: 35479414
- Cheng WL et al. ALK7 Acts as a Positive Regulator of Macrophage Activation through Down-Regulation of PPAR? Expression. J Atheroscler Thromb 28:375-384 (2021). PubMed: 32641645
- Wilkinson H et al. PAR-1 signaling on macrophages is required for effective in vivo delayed-type hypersensitivity responses. iScience 24:101981 (2021). PubMed: 33458623
- Gong FH et al. Attenuated macrophage activation mediated by microRNA-183 knockdown through targeting NR4A2. Exp Ther Med 21:300 (2021). PubMed: 33717243
- Han M et al. Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO Mol Med 12:e10924 (2020). PubMed: 31777202