Overview

  • Product name
  • Description
    Rabbit polyclonal to ABLIM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ABLIM1 aa 174-319.
    Database link: O14639

  • Positive control
    • WB: HeLa (human epithelial cell line from cervix adenocarcinoma) and HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates. ICC/IF: HepG2 (human liver hepatocellular carcinoma cell line) cells. IHC-P: Human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab222824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 87.7 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function
    May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
  • Tissue specificity
    Detected in liver, heart, skeletal muscle, brain and retina, where it is concentrated in the inner segment and in the outer plexiform layers.
  • Sequence similarities
    Contains 1 HP (headpiece) domain.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABLIM 1 antibody
    • ABLIM antibody
    • abLIM-1 antibody
    • ABLIM-L antibody
    • ABLIM-M antibody
    • ABLIM-S antibody
    • ABLIM1 antibody
    • ABLM1_HUMAN antibody
    • Actin binding double zinc finger protein antibody
    • Actin binding LIM protein 1 antibody
    • Actin binding LIM protein family member 1 antibody
    • Actin-binding double zinc finger protein antibody
    • Actin-binding LIM protein 1 antibody
    • Actin-binding LIM protein family member 1 antibody
    • DKFZp781D0148 antibody
    • FLJ14564 antibody
    • KIAA0059 antibody
    • LIM actin binding protein 1 antibody
    • LIMAB 1 antibody
    • LIMAB1 antibody
    • Limatin antibody
    • MGC1224 antibody
    • MGC125264 antibody
    • OTTHUMP00000020530 antibody
    • OTTHUMP00000020531 antibody
    • OTTHUMP00000020532 antibody
    • OTTHUMP00000020533 antibody
    • OTTHUMP00000020534 antibody
    • OTTHUMP00000020535 antibody
    • OTTHUMP00000020536 antibody
    • OTTHUMP00000020537 antibody
    • RGD1565768 antibody
    • RP11-317F20.1 antibody
    see all

Images

  • All lanes : Anti-ABLIM1 antibody (ab222824) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Mouse heart lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 87.7 kDa
    Observed band size: 88 kDa
    why is the actual band size different from the predicted?

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ABLIM1 (green) using ab222824 at a dilution of 1/100 in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin embedded sections of human heart tissue were stained for ABLIM1 with ab222824 at a dilution of 1/100.

References

ab222824 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab222824.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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