This antibody gave a positive signal in the following tissue lysates: Mouse Embryonic Brain E16; Mouse Olfactory Bulb; Mouse Hippocampus; Mouse Cerebellum.
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Actin binding LIM protein family member 3 antibody
Actin-binding LIM protein 3 antibody
Actin-binding LIM protein family member 3 antibody
Western blot - Anti-ABLIM3 antibody (ab129480)
All lanes : Anti-ABLIM3 antibody (ab129480) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : E16 Ms Embryo Brain Tissue Lysate Lane 2 : Mouse Olfactory Bulb Lysate Lane 3 : Mouse Hippocampus Tissue Lysate Lane 4 : Cerebellum Mouse Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa Observed band size: 78 kDa Additional bands at: 68 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent - 3%.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab129480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.