Product nameAnti-ABLIM3 antibody
See all ABLIM3 primary antibodies
DescriptionRabbit polyclonal to ABLIM3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human ABLIM3.
- This antibody gave a positive signal in the following tissue lysates: Mouse Embryonic Brain E16; Mouse Olfactory Bulb; Mouse Hippocampus; Mouse Cerebellum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). Abcam recommends using milk as the blocking agent - 3%|
FunctionMay act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
Tissue specificityExpressed predominantly in heart and brain.
Sequence similaritiesContains 1 HP (headpiece) domain.
Contains 4 LIM zinc-binding domains.
- Information by UniProt
- abLIM-3 antibody
- Ablim3 antibody
- ABLM3_HUMAN antibody
All lanes : Anti-ABLIM3 antibody (ab129480) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : E16 Ms Embryo Brain Tissue Lysate
Lane 2 : Mouse Olfactory Bulb Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : Cerebellum Mouse Tissue Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 68 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab129480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab129480 has not yet been referenced specifically in any publications.