Anti-AC5 antibody (ab66037)
Key features and details
- Rabbit polyclonal to AC5
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AC5 antibody -
Description
Rabbit polyclonal to AC5 -
Host species
Rabbit -
Specificity
This antibody can cross-react with ADCY6. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human AC5 (internal sequence).
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Positive control
- Extracts from COLO205 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa). |
Target
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Function
This is a membrane-bound, calcium-inhibitable adenylyl cyclase. -
Sequence similarities
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 111 Human
- Entrez Gene: 224129 Mouse
- Entrez Gene: 64532 Rat
- Omim: 600293 Human
- SwissProt: O95622 Human
- SwissProt: P84309 Mouse
- SwissProt: Q04400 Rat
- Unigene: 593292 Human
see all -
Alternative names
- AC 5 antibody
- AC5 antibody
- ADCY 5 antibody
see all
Images
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All lanes : Anti-AC5 antibody (ab66037) at 1/500 dilution
Lane 1 : Extracts from COLO205 cells with no immunising peptide
Lane 2 : Extracts from COLO205 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 139 kDa
Observed band size: 139 kDa -
ICC/IF image of ab66037 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66037, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab66037 has been referenced in 6 publications.
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Benavente F et al. Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair. Elife 9:N/A (2020). PubMed: 32894219
- Bravo-Sagua R et al. Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress. Cell Death Differ 26:1195-1212 (2019). PubMed: 30209302
- Romero-Calvo I et al. Adenylyl cyclase 6 is involved in the hyposecretory status of experimental colitis. Pflugers Arch 470:1705-1717 (2018). PubMed: 30094477
- Hodson DJ et al. ADCY5 couples glucose to insulin secretion in human islets. Diabetes 63:3009-21 (2014). IHC . PubMed: 24740569
- del Puerto A et al. Adenylate cyclase 5 coordinates the action of ADP, P2Y1, P2Y13 and ATP-gated P2X7 receptors on axonal elongation. J Cell Sci 125:176-88 (2012). Mouse . PubMed: 22250198