Overview

  • Product name
    Anti-ACAA2 antibody [EPR6732(2)]
    See all ACAA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6732(2)] to ACAA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human ACAA2 (P42765)

  • Positive control
    • HeLa, Caco-2 or HepG2 lysate, Human kidney tissue, HepG2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
    • Pathway
      Lipid metabolism; fatty acid metabolism.
    • Sequence similarities
      Belongs to the thiolase family.
    • Cellular localization
      Mitochondrion. Colocalizes with BNIP3 in the mitochondria.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 ketoacyl CoA thiolase antibody
      • 3 ketoacyl CoA thiolase mitochondrial antibody
      • 3-ketoacyl-CoA thiolase, mitochondrial antibody
      • 3-oxoacyl-CoA thiolase, mitochondrial antibody
      • ACAA 2 antibody
      • Acaa2 antibody
      • Acetyl CoA acyltransferase 2 antibody
      • Acetyl CoA acyltransferase antibody
      • Acetyl Coenzyme A acyltransferase 2 antibody
      • Acetyl-CoA acyltransferase antibody
      • Beta ketothiolase antibody
      • Beta-ketothiolase antibody
      • DSAEC antibody
      • Mitochondrial 3 oxoacyl CoA thiolase antibody
      • Mitochondrial 3 oxoacyl Coenzyme A thiolase antibody
      • Mitochondrial 3-oxoacyl-CoA thiolase antibody
      • T1 antibody
      • THIM_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ACAA2 antibody [EPR6732(2)] (ab128929) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Caco-2 cell lysate
      Lane 3 : HepG2 cel lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat

      anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • ab128929, at a 1/50 dilution, staining Human ACAA2 in kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
    • ab128929, at a 1/250 dilution, staining Human ACAA2 in HepG2 cells, using Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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