Overview

  • Product name
    Anti-ACTL8 antibody [EPR13912]
    See all ACTL8 primary antibodies
  • Description
    Rabbit monoclonal [EPR13912] to ACTL8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ACTL8 aa 200-300. The exact sequence is proprietary.
    Database link: Q9H568

  • Positive control
    • HeLa, HepG2 and Human testis lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13912
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40 - 1/60.

Target

  • Tissue specificity
    Strongly expressed in testis and pancreas. Weak expression in placenta.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin like 8 antibody
    • Actin like protein 8 antibody
    • Actin like protein antibody
    • Actin-like protein 8 antibody
    • ACTL8 antibody
    • ACTL8_HUMAN antibody
    • Cancer/testis antigen 57 antibody
    • CT57 antibody
    see all

Images

  • All lanes : Anti-ACTL8 antibody [EPR13912] (ab184562) at 1/2000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACTL8 with ab184562 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or PBS (lane 2) immunoprecipitated using ab184562 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

  • Anti-ACTL8 antibody [EPR13912] (ab184562) at 1/2000 dilution + Human testis lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa

References

This product has been referenced in:
  • Wu ZJ  et al. CRISPR/Cas9-mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation. Int J Oncol 52:1209-1223 (2018). Read more (PubMed: 29532865) »
See 1 Publication for this product

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