Anti-ACTL8 antibody (ab96756)
Key features and details
- Rabbit polyclonal to ACTL8
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACTL8 antibody
See all ACTL8 primary antibodies -
Description
Rabbit polyclonal to ACTL8 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 156 and 353 of ACTL8 (NP_110439)
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Positive control
- A431 and Molt-4 whole cell lysate. H1299, HeLa, HepG2 cell lines. A549 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested.
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Notes |
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ICC/IF
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested. |
Target
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Tissue specificity
Strongly expressed in testis and pancreas. Weak expression in placenta. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 81569 Human
- GenBank: NM_030812 Human
- SwissProt: Q9H568 Human
- Unigene: 2149 Human
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Alternative names
- Actin like 8 antibody
- Actin like protein 8 antibody
- Actin like protein antibody
see all
Images
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All lanes : Anti-ACTL8 antibody (ab96756) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa -
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ab96756 at 1/500 dilution.
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ab96756 at 1/200 staining ACTL8 in methanol-fixed HeLa by Immunofluorescence, lower image is costained with Hoeschst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab96756 has been referenced in 2 publications.
- Ma S et al. Actin-like protein 8 promotes cell proliferation, colony-formation, proangiogenesis, migration and invasion in lung adenocarcinoma cells. Thorac Cancer 11:526-536 (2020). PubMed: 31962007
- Ma S et al. Knockdown of actin-like 8 inhibits cell proliferation by regulating FOXM1, STMN1, PLK1, and BIRC5 in lung adenocarcinoma A549 cells. Transl Cancer Res 8:1975-1984 (2019). PubMed: 35116946