Anti-ADAMTS4 antibody (ab185722)
Key features and details
- Rabbit polyclonal to ADAMTS4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS4 antibody
See all ADAMTS4 primary antibodies -
Description
Rabbit polyclonal to ADAMTS4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human ADAMTS4 aa 50-350.
Database link: O75173 -
Positive control
- WB: SH-SY5Y, A-549, SKOV3, 293T cell lysates, mouse and brain tissue lysates. ICC/ IF: C6 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 90 kDa.
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ICC/IF | (1) |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 90 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site. -
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 286806 Cow
- Entrez Gene: 9507 Human
- Entrez Gene: 240913 Mouse
- Entrez Gene: 100461178 Orangutan
- Entrez Gene: 66015 Rat
- Omim: 603876 Human
- SwissProt: Q9TT93 Cow
- SwissProt: O75173 Human
see all -
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
see all
Images
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Immunofluorescence staining of C6 cells stained for ADAMTS4 with ab185722 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-ADAMTS4 antibody (ab185722) at 1/3000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : A-549 cell lysate
Lane 3 : SKOV3 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Rat brain tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 30 secondsBlocking buffer: 3% nonfat dry milk in TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (59)
ab185722 has been referenced in 59 publications.
- Kaufmann JO et al. ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries. Nat Commun 13:2867 (2022). PubMed: 35606349
- Abu El-Asrar AM et al. Differential Expression and Localization of ADAMTS Proteinases in Proliferative Diabetic Retinopathy. Molecules 27:N/A (2022). PubMed: 36144730
- Zhang J et al. Knockdown of triggering receptor expressed on myeloid cells 1 (TREM1) inhibits endoplasmic reticulum stress and reduces extracellular matrix degradation and the apoptosis of human nucleus pulposus cells. Exp Ther Med 24:607 (2022). PubMed: 36160910
- Zheng X et al. Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment. Front Pharmacol 13:938133 (2022). PubMed: 36238555
- Kement D et al. Neuroserpin Is Strongly Expressed in the Developing and Adult Mouse Neocortex but Its Absence Does Not Perturb Cortical Lamination and Synaptic Proteome. Front Neuroanat 15:627896 (2021). PubMed: 33708076