Anti-ADAMTS5 antibody (ab182795)
Key features and details
- Rabbit polyclonal to ADAMTS5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS5 antibody
See all ADAMTS5 primary antibodies -
Description
Rabbit polyclonal to ADAMTS5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ADAMTS5 aa 500-600.
Database link: Q9UNA0 -
Positive control
- WB: Human fetal brain tissue. IHC-P: Human lung, tonsil and kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/10 - 1/50.
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WB |
1/500 - 1/2000. Predicted molecular weight: 102 kDa.
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Notes |
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IHC-P
1/10 - 1/50. |
WB
1/500 - 1/2000. Predicted molecular weight: 102 kDa. |
Target
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. -
Tissue specificity
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 11096 Human
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- Unigene: 58324 Human
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded human lung tissue labeling ADAMTS5 with ab182795 at 1/50 dilution.
The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin embedded human kidney tissue labeling ADAMTS5 with ab182795 at 1/50 dilution.
The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling ADAMTS5 with ab182795 at 1/10. Image on the right is treated with the synthetic peptide.
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Anti-ADAMTS5 antibody (ab182795) at 1/650 dilution + Human fetal brain tissue at 40 µg
Secondary
Goat anti Rabbit IgG at 1/3000 dilution
Predicted band size: 102 kDa
Exposure time: 3 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab182795 has been referenced in 9 publications.
- Xu H et al. Col9a2 gene deletion accelerates the degeneration of intervertebral discs. Exp Ther Med 23:207 (2022). PubMed: 35126710
- Zhang J et al. Hypoxia-inducible factor expression is related to apoptosis and cartilage degradation in temporomandibular joint osteoarthritis. BMC Musculoskelet Disord 23:583 (2022). PubMed: 35710352
- Hurley MM et al. FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms. Sci Rep 12:15968 (2022). PubMed: 36153352
- Duan A et al. Extracellular vesicles derived from LPS-preconditioned human synovial mesenchymal stem cells inhibit extracellular matrix degradation and prevent osteoarthritis of the knee in a mouse model. Stem Cell Res Ther 12:427 (2021). PubMed: 34321073
- Han J et al. β-Ecdysone attenuates cartilage damage in a mouse model of collagenase-induced osteoarthritis via mediating FOXO1/ADAMTS-4/5 signaling axis. Histol Histopathol 36:785-794 (2021). PubMed: 33876419
- Duan A et al. 1,25-Dihydroxyvitamin D Inhibits Osteoarthritis by Modulating Interaction Between Vitamin D Receptor and NLRP3 in Macrophages. J Inflamm Res 14:6523-6542 (2021). PubMed: 34887675
- Jing X et al. Iron Overload Is Associated With Accelerated Progression of Osteoarthritis: The Role of DMT1 Mediated Iron Homeostasis. Front Cell Dev Biol 8:594509 (2020). PubMed: 33469535
- Li Y et al. Artesunate alleviates interleukin-1ß-induced inflammatory response and apoptosis by inhibiting the NF-?B signaling pathway in chondrocyte-like ATDC5 cells, and delays the progression of osteoarthritis in a mouse model. Int J Mol Med 44:1541-1551 (2019). PubMed: 31364719
- Maudens P et al. Nanocrystal-Polymer Particles: Extended Delivery Carriers for Osteoarthritis Treatment. Small 14:N/A (2018). PubMed: 29327460