Overview

  • Product name
  • Description
    Rabbit polyclonal to ADH1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide within Human ADH1B aa 216-245 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P00325

  • Positive control
    • Recombinant Human ADH1B protein (ab116934) can be used as a positive control in WB. Human hepatocarcinoma tissue, HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Sequence similarities
    Belongs to the zinc-containing alcohol dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH beta subunit antibody
    • ADH1B antibody
    • ADH1B_HUMAN antibody
    • ADH2 antibody
    • Alcohol dehydrogenase 1B antibody
    • alcohol dehydrogenase 2 (class I), beta polypeptide antibody
    • Alcohol dehydrogenase 2 antibody
    • Alcohol dehydrogenase subunit beta antibody
    • Aldehyde reductase antibody
    • DKFZp686C06125 antibody
    • OTTHUMP00000220192 antibody
    see all

Images

  • Immunofluorescence analysis of HepG2 cells labeling ADH1B with ab175515 at a 1/10 dilution, followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Anti-ADH1B antibody (ab175515) at 1/1000 dilution + HepG2 cell line lysate at 35 µg

    Secondary
    Anti-rabbit HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin-embedded Human hepatocarcinoma tissue labeling ADH1B with ab175515 at a 1/50 dilution, followed by DAB staining.

  • Flow cytometry analysis of HepG2 cells labeling ADH1B with ab175515 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175515 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175515.
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