Product nameAnti-ADH1B antibody
See all ADH1B primary antibodies
DescriptionRabbit polyclonal to ADH1B
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
Synthetic peptide within Human ADH1B aa 216-245 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P00325
- Recombinant Human ADH1B protein (ab116934) can be used as a positive control in WB. Human hepatocarcinoma tissue, HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab175515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 40 kDa.|
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
- Information by UniProt
- ADH beta subunit antibody
- ADH1B antibody
- ADH1B_HUMAN antibody
Immunofluorescence analysis of HepG2 cells labeling ADH1B with ab175515 at a 1/10 dilution, followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Anti-ADH1B antibody (ab175515) at 1/1000 dilution + HepG2 cell line lysate at 35 µg
Anti-rabbit HRP conjugated antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human hepatocarcinoma tissue labeling ADH1B with ab175515 at a 1/50 dilution, followed by DAB staining.
Flow cytometry analysis of HepG2 cells labeling ADH1B with ab175515 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175515 has not yet been referenced specifically in any publications.