Overview

  • Product name

    AGE-BSA
  • Protein length

    Full length protein
  • Description

    Native AGE protein

Description

  • Nature

    Native
  • Source

    Native

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level

    < 0.100 Eu/µg
  • Purity

    > 98 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    May precipitate during extended storage. Sonicate to solubilize the precipitates. The AGE modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps. Fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex./Em. = 370/440 nm. Glycated BSA shows a 7000% increase in fluorescence in compared to control BSA.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Constituent: PBS

General Info

  • Alternative names

    • Advanced glycation end products
    • AGEs
  • Relevance

    The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
  • Cellular localization

    Cell Membrane and Secreted

Images

  • 4-20% SDS-PAGE of AGE-BSA: 10, 15 and 20 ug of AGE-BSA loaded in each lane under reducing conditions and stained with Coomassie Blue. AGE-BSA has a predicted MW of ~ 66.5 kDa

References

This product has been referenced in:

  • Di B  et al. Liraglutide inhibited AGEs induced coronary smooth muscle cell phenotypic transition through inhibiting the NF-?B signal pathway. Peptides 112:125-132 (2019). Read more (PubMed: 30513352) »
  • Krishnamoorthy D  et al. Dietary advanced glycation end-product consumption leads to mechanical stiffening of murine intervertebral discs. Dis Model Mech 11:N/A (2018). Read more (PubMed: 30498097) »
See all 4 Publications for this product

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