Overview

  • Product name
  • Description
    Rabbit polyclonal to AIDA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human AIDA aa 125-192.
    Sequence:

    EEENLEFEEDEEEGGAGAGSPDSFPARVPGTLLPRLPSEPGMTLLTIRIE KIGLKDAGQCIDPYITVS


    Database link: Q96BJ3-1

  • Positive control
    • IHC-P: Human cerebellum tissue. ICC/IF: A431 cells. WB: U-251 MG cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab251782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 35 kDa.

Target

  • Function
    Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
  • Tissue specificity
    Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
  • Sequence similarities
    Belongs to the AIDA family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610208M17Rik antibody
    • AIDA antibody
    • AIDA_HUMAN antibody
    • Axin interaction partner and dorsalization antagonist antibody
    • Axin interactor antibody
    • Axin interactor dorsalization associated protein antibody
    • Axin interactor, dorsalization associated antibody
    • C1orf80 antibody
    • Chromosome 1 open reading frame 80 antibody
    • dorsalization-associated protein antibody
    • RP11-378J18.7 antibody
    • UPF0491 protein C1orf80 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for AIDA (green) using ab251782 at 4 µg/ml in ICC/IF.
  • Paraffin-embedded human cerebellum tissue stained for AIDA using ab251782 at 1/150 dilution in immunohistochemical analysis.
  • All lanes : Anti-AIDA antibody (ab251782) at 0.4 µg/ml

    Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate

    Predicted band size: 35 kDa

References

ab251782 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab251782.
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