Anti-AKR1C1 antibody (ab192785)
Key features and details
- Rabbit polyclonal to AKR1C1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-AKR1C1 antibody
See all AKR1C1 primary antibodies -
Description
Rabbit polyclonal to AKR1C1 -
Host species
Rabbit -
Specificity
This antibody cross-reacts with AKR1C2.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human AKR1C1 aa 22-248. The exact sequence is proprietary.
Database link: Q04828 -
Positive control
- Human liver tissue; A431 cells; Raji whole cell lysate; mouse liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Constituents: 20% Glycerol (glycerin, glycerine), PBS, 0.00038% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00038% 2-methyl-4-isothiazolin-3-one -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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IHC-P |
Use a concentration of 7.5 µg/ml.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
IHC-P
Use a concentration of 7.5 µg/ml. |
Target
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Function
Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. -
Tissue specificity
Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1645 Human
- Omim: 600449 Human
- SwissProt: Q04828 Human
- Unigene: 460260 Human
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Alternative names
- 2-dihydrobenzene-1 antibody
- 2-diol dehydrogenase antibody
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
see all
Images
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Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 37 kDa10% SDS PAGE
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Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + mouse liver tissue lysate at 50 µg
Predicted band size: 37 kDa10% SDS-PAGE
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Immunofluorescence analysis of A431 cells, labeling AKR1C1 (green, upper panel) with ab192785 at 1/200 dilution. Nuclei were counterstained with Hoechst 33342 (blue, lower panel).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue, labeling AKR1C1 with ab192785 at 7.5 μg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab192785 has been referenced in 4 publications.
- Zhang X et al. Glutathione Peroxidase 4 as a Therapeutic Target for Anti-Colorectal Cancer Drug-Tolerant Persister Cells. Front Oncol 12:913669 (2022). PubMed: 35719967
- Nie J et al. A Novel Ferroptosis Related Gene Signature for Prognosis Prediction in Patients With Colon Cancer. Front Oncol 11:654076 (2021). PubMed: 34046350
- Narzt MS et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. Redox Biol 20:467-482 (2019). PubMed: 30466060
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201