Recombinant
RabMAb

Recombinant Anti-ALDH16A1 antibody [EPR9217] (ab137082)

Overview

  • Product name
    Anti-ALDH16A1 antibody [EPR9217]
    See all ALDH16A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9217] to ALDH16A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ALDH16A1 aa 550-650. The exact sequence is proprietary.

  • Positive control
    • HeLa, Jurkat, 293T and HepG2 cell lysates; and Human colon and hepatocellular carcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9217
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance
      This gene encodes a member of the aldehyde dehydrogenase superfamily. The family members act on aldehyde substrates and use nicotinamide adenine dinucleotide phosphate (NADP) as a cofactor. This gene is conserved in chimpanzee, dog, cow, mouse, rat, and zebrafish. The protein encoded by this gene interacts with maspardin, a protein that when truncated is responsible for Mast syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
    • Database links
    • Alternative names
      • Aldehyde dehydrogenase 16 family, member A1 antibody
      • Aldehyde dehydrogenase family 16 member A1 antibody
      • ALDH16A1 aldehyde dehydrogenase 16 family, member A1 antibody
      • Aldh16a1 antibody
      • MGC10204 antibody
      see all

    Images

    • All lanes : Anti-ALDH16A1 antibody [EPR9217] (ab137082) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : 293T cell lysates
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 85 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ALDH16A1 with ab137082 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling ALDH16A1 with ab137082 at 1/50 dilution.

    References

    ab137082 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137082.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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