Anti-AMBRA1 antibody (ab69501)
Key features and details
- Rabbit polyclonal to AMBRA1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AMBRA1 antibody
See all AMBRA1 primary antibodies -
Description
Rabbit polyclonal to AMBRA1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human AMBRA1 (C terminal). AMBRA1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human AMBRA1 .
Database link: Q9C0C7 -
Positive control
- WB: NIH/3T3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa). |
Target
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Function
Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. -
Sequence similarities
Contains 3 WD repeats. -
Cellular localization
Cytoplasmic vesicle > autophagosome. - Information by UniProt
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Database links
- Entrez Gene: 55626 Human
- Entrez Gene: 228361 Mouse
- Omim: 611359 Human
- SwissProt: Q9C0C7 Human
- SwissProt: A2AH22 Mouse
- Unigene: 654644 Human
- Unigene: 436667 Mouse
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Alternative names
- Activating molecule in beclin 1 regulated autophagy antibody
- Activating molecule in BECN1 regulated autophagy protein 1 antibody
- Activating molecule in BECN1-regulated autophagy protein 1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab69501 has been referenced in 2 publications.
- Siracusa R et al. Autophagy and Mitophagy Promotion in a Rat Model of Endometriosis. Int J Mol Sci 22:N/A (2021). PubMed: 34064854
- Kim JH et al. Protective Effects of Evogliptin on Steatohepatitis in High-Fat-Fed Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32937958