Overview

  • Product name
    Anti-ANKS1B antibody - C-terminal
    See all ANKS1B primary antibodies
  • Description
    Rabbit polyclonal to ANKS1B - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 182-231 (ACAKMRANCQ KSTEQMKKVP TIILSVSYKG VKFIDATNKN IIAEHEIRNI) of Human ANKS1B (NP_001190995).

  • Positive control
    • 293T cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab136310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.
    Isoform 3 can regulate global protein synthesis by altering nucleolar numbers.
    Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing.
  • Tissue specificity
    Highly expressed in marrow from patients with pre-B ALL associated with the t(1;19) translocation. Strongly expressed in brain and testis. Expressed in fetal brain. Isoform 4 is highly expressed in brain (at protein level). Isoform 6 is expressed in brain and several cancer cell lines.
  • Sequence similarities
    Contains 7 ANK repeats.
    Contains 1 PID domain.
    Contains 2 SAM (sterile alpha motif) domains.
  • Post-translational
    modifications
    Isoform 3 nuclear translocation requires an NMDAR-dependent proteolytic cleavage.
  • Cellular localization
    Cytoplasm; Nucleus; Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendritic spine. Nucleus. Nucleus > Cajal body. The synaptic localization requires DLG4 interaction. Translocation to the nucleus in response to stimulation of NMDA receptors (NMDARs) in a calcium-independent manner and Nucleus. The interaction with APP causes its partial exclusion from the nucleus, when APP is overexpressed.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIDA antibody
    • AIDA-1 antibody
    • Amyloid-beta protein intracellular domain-associated protein 1 antibody
    • Anks1b antibody
    • ANKS2 antibody
    • Ankyrin repeat and sterile alpha motif domain containing 1B antibody
    • Ankyrin repeat and sterile alpha motif domain-containing protein 1B antibody
    • ANS1B_HUMAN antibody
    • Cajalin 2 antibody
    • E2A-PBX1-associated protein antibody
    • EB-1 antibody
    • EB1 antibody
    • MGC26087 antibody
    see all

Images

  • IHC image of ANKS1B staining in a section of formalin-fixed paraffin-embedded normal Human hippocampus* performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab136310, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-ANKS1B antibody - C-terminal (ab136310) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    Goat anti-rabbit IgG HRP

    Developed using the ECL technique.

    Predicted band size: 40 kDa

References

ab136310 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - other (Neurons-Synaptosomes)
Loading amount
30 µg
Specification
Neurons-Synaptosomes
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Bryen A. Jordan

Verified customer

Submitted Mar 11 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up