Recombinant Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20910-105] to ANO6 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free
See all ANO6 primary antibodies -
Description
Rabbit monoclonal [EPR20910-105] to ANO6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
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General notes
ab256302 is the carrier-free version of ab234422.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20910-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 106 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 106 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Function
May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system. -
Tissue specificity
Expressed in embryonic stem cell, fetal liver, retina, chronic myologenous leukemia and intestinal cancer. -
Involvement in disease
Defects in ANO6 are the cause of Scott syndrome (SCOTTS) [MIM:262890]. A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents. -
Sequence similarities
Belongs to the anoctamin family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 105722 Mouse
- Entrez Gene: 315272 Rat
- SwissProt: Q6P9J9 Mouse
- Unigene: 38087 Mouse
- Unigene: 392547 Mouse
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Alternative names
- 2900059G15Rik antibody
- AA407480 antibody
- Ano6 antibody
see all
Images
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ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Mouse spleen lysate 10 µg (Input).
Lane 2: ab234422 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234422).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab256302 has not yet been referenced specifically in any publications.