Recombinant Anti-ANO6 antibody [EPR20910-105] (ab234422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20910-105] to ANO6
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ANO6 antibody [EPR20910-105]
See all ANO6 primary antibodies -
Description
Rabbit monoclonal [EPR20910-105] to ANO6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20910-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 106 kDa.
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IP |
1/30.
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Notes |
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WB
1/1000. Predicted molecular weight: 106 kDa. |
IP
1/30. |
Target
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Function
May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system. -
Tissue specificity
Expressed in embryonic stem cell, fetal liver, retina, chronic myologenous leukemia and intestinal cancer. -
Involvement in disease
Defects in ANO6 are the cause of Scott syndrome (SCOTTS) [MIM:262890]. A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents. -
Sequence similarities
Belongs to the anoctamin family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 105722 Mouse
- Entrez Gene: 315272 Rat
- SwissProt: Q6P9J9 Mouse
- Unigene: 38087 Mouse
- Unigene: 392547 Mouse
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Alternative names
- 2900059G15Rik antibody
- AA407480 antibody
- Ano6 antibody
see all
Images
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All lanes : Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 120-250 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with literatures (PMID: 29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 37 secs; Lane 3: 124 secs; Lane 4: 70 secs.
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ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Mouse spleen lysate 10 µg (Input).
Lane 2: ab234422 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab234422 has not yet been referenced specifically in any publications.