Anti-AP3B2 antibody (ab48269)
Key features and details
- Rabbit polyclonal to AP3B2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AP3B2 antibody
See all AP3B2 primary antibodies -
Description
Rabbit polyclonal to AP3B2 -
Host species
Rabbit -
Specificity
By Western blot this antibody detects a ~119-130 kDa protein representing AP3B2 from brain samples. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human AP3B2 aa 260-274. Mouse sequence is LLEENPEKAFYGSEE
Sequence:LLEENAEKAFYGSEE
(Peptide available asab48404) -
Positive control
- Human brain samples.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 119-130 kDa (predicted molecular weight: 119 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 119-130 kDa (predicted molecular weight: 119 kDa). |
Target
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Function
Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. -
Tissue specificity
Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). -
Sequence similarities
Belongs to the adaptor complexes large subunit family. -
Developmental stage
Expressed from early in development through adulthood. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Golgi apparatus. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 8120 Human
- Entrez Gene: 11775 Mouse
- Entrez Gene: 308777 Rat
- Omim: 602166 Human
- SwissProt: Q13367 Human
- SwissProt: Q9JME5 Mouse
- Unigene: 199593 Human
- Unigene: 322894 Mouse
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Alternative names
- Adapter-related protein complex 3 subunit beta-2 antibody
- Adaptor protein complex AP-3 subunit beta-2 antibody
- Adaptor related protein complex 3 beta 2 subunit antibody
see all
Images
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Anti-AP3B2 antibody (ab48269) at 1/500 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
A ~50 kDa band is also seen in Rat and Mouse brain tissue, representing the medium chain homolog (p47A) of AP3.
Datasheets and documents
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Datasheet download
References (1)
ab48269 has been referenced in 1 publication.
- Ku YC et al. The transcriptome of utricle hair cell regeneration in the avian inner ear. J Neurosci 34:3523-35 (2014). PubMed: 24599453