Overview

  • Product name
    APC Conjugation Kit
  • Product overview

    Abcam APC Conjugation Kit (ab201807) uses a simple and quick process to conjugate an antibody to APC. It can also be used to conjugate other proteins or peptides.


    To conjugate an antibody to APC using this kit:
    - add modifier to antibody and incubate for 3 hours
    - add quencher and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to APC.

  • Notes

    Amount and volume of antibody for conjugation to APC

     Kit size   Recommended 
    maximum amount of antibody
     
    Maximum antibody 
    volume
    30 µg   3 x 10 µg 3 x 10 µL
    300 µg   3 x 100 µg  3 x 100 µL
    1 mg   1 mg 1 mL

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1%/1% BSA2 50% glycerol
    0.1% sodium azide PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.

    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 µg 300 µg 30 µg 1 mg
    APC Conjugation Mix 1 vial 3 vials 3 vials 1 vial
    APC Modifier Reagent 1 vial 1 vial 1 vial 1 vial
    APC Quencher Reagent 1 vial 1 vial 1 vial 1 vial
  • Research areas
  • Alternative names
    • Allophycocyanin

Applications

Our Abpromise guarantee covers the use of ab201807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

ab201807 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201807.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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