Anti-AS160 (phospho T642) antibody (ab65753)
Key features and details
- Sheep polyclonal to AS160 (phospho T642)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-AS160 (phospho T642) antibody
See all AS160 primary antibodies -
Description
Sheep polyclonal to AS160 (phospho T642) -
Host species
Sheep -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AS160 aa 600-700 conjugated to keyhole limpet haemocyanin.
Database link: O60343 -
Positive control
- WB: HEK-293 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa.
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Notes |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa. |
Target
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Function
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. -
Tissue specificity
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis. -
Sequence similarities
Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. -
Cellular localization
Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. - Information by UniProt
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Database links
- Entrez Gene: 9882 Human
- Omim: 612465 Human
- SwissProt: O60343 Human
- Unigene: 210891 Human
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Alternative names
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
see all
Images
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All lanes : Anti-AS160 (phospho T642) antibody (ab65753) at 2 µg/ml
Lane 1 : AS160 wild type (WT)
Lane 2 : AS160 wild type (WT) with (+) 1 µM insulin treatment for 15 minutes
Lane 3 : S588A mutant (S588A)
Lane 4 : S588A mutant (S588A) with (+) 1 µM insulin treatment for 15 minutes
Lane 5 : T642A mutant (T642A)
Lane 6 : T642A mutant (T642A) with (+) 1 µM insulin treatment for 15 minutes
Predicted band size: 147 kDaWestern blot analysis of immunoprecipitated Flagtagged AS160 wild type (WT), S588A mutant (S588A), and T642A mutant (T642A) over-expressed transiently in HEK-293 cells, basal (-) or with (+) 1 µM insulin treatment for 15 minutes.
Datasheets and documents
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Datasheet download
References (7)
ab65753 has been referenced in 7 publications.
- Ramos PA et al. Insulin-Stimulated Muscle Glucose Uptake and Insulin Signaling in Lean and Obese Humans. J Clin Endocrinol Metab 106:e1631-e1646 (2021). PubMed: 33382888
- Actis Dato V et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892
- Lu R et al. Protective role of Astragaloside IV in chronic glomerulonephritis by activating autophagy through PI3K/AKT/AS160 pathway. Phytother Res 34:3236-3248 (2020). PubMed: 32726508
- Yang GS et al. mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild hypothermia. Mol Med Rep 17:5821-5829 (2018). PubMed: 29484389
- Krag TO et al. Differential glucose metabolism in mice and humans affected by McArdle disease. Am J Physiol Regul Integr Comp Physiol 311:R307-14 (2016). PubMed: 27280431
- Inazuka F et al. Muscle-specific Knock-out of NUAK Family SNF1-like Kinase 1 (NUAK1) Prevents High Fat Diet-induced Glucose Intolerance. J Biol Chem 287:16379-89 (2012). WB ; Mouse . PubMed: 22418434
- Schweitzer GG et al. Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation. J Appl Physiol 113:1852-61 (2012). WB ; Rat . PubMed: 22936728