Anti-ATP5C1 antibody (ab190875)
Key features and details
- Goat polyclonal to ATP5C1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-ATP5C1 antibody
See all ATP5C1 primary antibodies -
Description
Goat polyclonal to ATP5C1 -
Host species
Goat -
Specificity
ab190875 is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1). The immunizing peptide represents the N terminus of the mature protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Cow, Dog, Human, Cynomolgus monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ATP5C1 aa 1-100 (internal sequence) (Cysteine residue). (NP_001001973.1).
Database link: P36542 -
Positive control
- Rat skeletal muscle lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab190875 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
Tissue specificity
Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta. -
Sequence similarities
Belongs to the ATPase gamma chain family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 327668 Cow
- Entrez Gene: 102133224 Cynomolgus monkey
- Entrez Gene: 478009 Dog
- Entrez Gene: 509 Human
- Entrez Gene: 11949 Mouse
- Entrez Gene: 100447834 Orangutan
- Entrez Gene: 116550 Rat
- Omim: 108729 Human
see all -
Alternative names
- ATP synthase gamma chain, mitochondrial antibody
- ATP synthase subunit gamma antibody
- ATP synthase subunit gamma, mitochondrial antibody
see all
Images
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Anti-ATP5C1 antibody (ab190875) at 0.1 µg/ml + Rat skeletal muscle lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab190875 has not yet been referenced specifically in any publications.