Product nameAnti-ATP5F1 antibody [9D1BC4]
See all ATP5F1 primary antibodies
DescriptionMouse monoclonal [9D1BC4] to ATP5F1
Tested applicationsSuitable for: IHC-P, WB, ICC, In-Cell ELISA, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Purified human liver mitochondria
- Human normal colon FFPE tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Light chain typekappa
Our Abpromise guarantee covers the use of ab117991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 24.6 kDa.|
|ICC||Use a concentration of 1 µg/ml.|
|In-Cell ELISA||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Sequence similaritiesBelongs to the eukaryotic ATPase B chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- AT5F1_HUMAN antibody
- ATP synthase B chain mitochondrial antibody
- ATP synthase subunit b antibody
All lanes : Anti-ATP5F1 antibody [9D1BC4] (ab117991) at 1 µg/ml
Lane 1 : Human heart homogenate at 15 µg
Lane 2 : HepG2 lysate at 15 µg
Lane 3 : Human liver mitochondria at 7.5 µg
Lane 4 : Bovine heart mitochondria at 7.5 µg
Lane 5 : Rat liver mitochondria at 7.5 µg
Lane 6 : Mouse liver mitochondria at 7.5 µg
Predicted band size: 24.6 kDa
Note - Extra bands seen in lane 6 are secondary antibody mouse-on-mouse effects and not related to the primary antibody.
ab117991 at 1 ug/ml with Hela cells in flow cytometry.
Immunocytochemistry image of ab117991 (MS972) stained NIH3T3 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with urea/heat antigen retrieval method. The cells were incubated with ab117991 at 1 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the mitochondria. The four cells in the upper portion of the image show mitochondria in the elongated, reticular, arrangement, the three cells in the lower portion of the image show a punctuate mitochondrial organization and may be dividing/have recently divided.
IHC image of ATP5F1 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117991, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Ihara M et al. An interaction between glucagon-like peptide-1 and adenosine contributes to cardioprotection of a dipeptidyl peptidase 4 inhibitor from myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol 308:H1287-97 (2015). Read more (PubMed: 25747753) »
- Kimura T et al. Autophagy protects kidney proximal tubule epithelial cells from mitochondrial metabolic stress. Autophagy 9:1876-86 (2013). Read more (PubMed: 24128672) »