Recombinant Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (ab249327)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11326(B)] to ATP6V1D - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free
See all ATP6V1D primary antibodies -
Description
Rabbit monoclonal [EPR11326(B)] to ATP6V1D - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra), IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249327 is the carrier-free version of ab157458.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11326(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)
- Alexa Fluor® 488 Anti-ATP6V1D antibody [EPR11326(B)] (ab309728)
- Alexa Fluor® 647 Anti-ATP6V1D antibody [EPR11326(B)] (ab310094)
- Alexa Fluor® 594 Anti-ATP6V1D antibody [EPR11326(B)] (ab310498)
- Alexa Fluor® 555 Anti-ATP6V1D antibody [EPR11326(B)] (ab312027)
- Alexa Fluor® 568 Anti-ATP6V1D antibody [EPR11326(B)] (ab312504)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. -
Cellular localization
Cell Membrane and Cytoplasmic -
Database links
- Entrez Gene: 51382 Human
- Entrez Gene: 73834 Mouse
- Entrez Gene: 299159 Rat
- SwissProt: Q9Y5K8 Human
- SwissProt: P57746 Mouse
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Alternative names
- ATP6M antibody
- ATP6V1D antibody
- V-ATPase 28 kDa accessory protein antibody
see all
Images
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All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab157458, the same antibody clone in a different buffer formulation.
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This data was developed using ab157458, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab157458, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab157458, the same antibody clone in a different buffer formulation.
Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.
Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution + Immunoprecipitation pellet from Y79 cell lysate at 10 µg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249327 has not yet been referenced specifically in any publications.