Anti-ATP6V1E1 antibody (ab111733)
Key features and details
- Rabbit polyclonal to ATP6V1E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ATP6V1E1 antibody
See all ATP6V1E1 primary antibodies -
Description
Rabbit polyclonal to ATP6V1E1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment within Human ATP6V1E1 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P36543 -
Positive control
- A549 and Mouse brain whole cell lysates; HeLa cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
|
|
ICC/IF |
1/100 - 1/500.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/500. |
Target
-
Relevance
Function: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Tissue specificity: Ubiquitous. Similarity: Belongs to the V-ATPase E subunit family. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 287017 Cow
- Entrez Gene: 529 Human
- Entrez Gene: 8992 Human
- Entrez Gene: 11973 Mouse
- Entrez Gene: 11974 Mouse
- Entrez Gene: 297566 Rat
- Entrez Gene: 94170 Rat
- Omim: 108746 Human
see all -
Alternative names
- ATP6E antibody
- ATP6E2 antibody
- ATP6V1E antibody
see all
Images
-
Anti-ATP6V1E1 antibody (ab111733) at 1/3000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-ATP6V1E1 antibody (ab111733) at 1/1000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunofluorescence analysis of ATP6V1E1 in methanol-fixed HeLa cells, using ab111733 at a 1/200 dilution. Lower image shows the cells counter stained with Hoechst 33342.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab111733 has been referenced in 6 publications.
- Pottie L et al. Loss of zebrafish atp6v1e1b, encoding a subunit of vacuolar ATPase, recapitulates human ARCL type 2C syndrome and identifies multiple pathobiological signatures. PLoS Genet 17:e1009603 (2021). PubMed: 34143769
- Ponsford AH et al. Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. Autophagy N/A:1-19 (2020). PubMed: 32515674
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). PubMed: 29652249
- Doyle T et al. The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nat Microbiol 3:1369-1376 (2018). PubMed: 30478388
- Cao X et al. V-ATPase promotes transforming growth factor-ß-induced epithelial-mesenchymal transition of rat proximal tubular epithelial cells. Am J Physiol Renal Physiol 302:F1121-32 (2012). WB, ICC/IF ; Rat . PubMed: 22129967
- Sarkar J et al. V-type ATPase proton pump expression during enamel formation. Matrix Biol 52-54:234-245 (0). PubMed: 26586472