Anti-Abhd5/CGI-58 antibody (ab111984)
Key features and details
- Goat polyclonal to Abhd5/CGI-58
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Abhd5/CGI-58 antibody
See all Abhd5/CGI-58 primary antibodies -
Description
Goat polyclonal to Abhd5/CGI-58 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human Abhd5/CGI-58 aa 150-250 (internal sequence).
Database link: Q8WTS1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 39 kDa.
|
|
IHC-P |
Use a concentration of 3.75 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 39 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
-
Function
Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. -
Tissue specificity
Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level). -
Involvement in disease
Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma. -
Sequence similarities
Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. -
Developmental stage
Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level). -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA. - Information by UniProt
-
Database links
- Entrez Gene: 485570 Dog
- Entrez Gene: 51099 Human
- Entrez Gene: 67469 Mouse
- Entrez Gene: 497624 Pig
- Entrez Gene: 316122 Rat
- Omim: 604780 Human
- SwissProt: Q8WTS1 Human
- SwissProt: Q9DBL9 Mouse
see all -
Alternative names
- 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
- ABHD5 antibody
- ABHD5_HUMAN antibody
see all
Images
-
ab111984, at 3.75 µg/ml, staining Abhd5/CGI-58 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
-
ab111984, at 3.75 µg/ml, staining Abhd5/CGI-58 in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.
-
Anti-Abhd5/CGI-58 antibody (ab111984) at 0.2 µg/ml + NIH3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Primary incubation was 1 hour.
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab111984 has been referenced in 1 publication.
- Gray NE et al. Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes. J Biol Chem 287:8444-56 (2012). WB ; Mouse . PubMed: 22267746