Product nameAcetyltransferase Activity Assay Kit (Fluorometric)
Sample typePurified protein, Inhibitor compounds
Acetyltransferase Activity Assay Kit (Fluorometric) (ab204536) is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.
This assay is a complete kit for the screening of candidate compounds that can alter normal acetyltransferase activity. For use with purified in vitro samples.
Acetylation is an important covalent molecular modification. Originally identified as the method by which certain bacteria were able to deactivate anti-microbial compounds, acetylation is now also known as an important partitioning and signaling modification.
Acetyltransferases are enzymes that covalently transfer an acetyl group from a donor molecule (Acetyl CoA) to an acceptor. Acetyl CoA serves as a universal donor while the acceptor varies with the acetyltransferase. Acceptors include histones, kinases, transcription factors, receptors, neurotransmitter precursors like choline and serotonin, and anti-microbial agents like chloramphenicol and fluoroquinones. Acetylation can signal an increase or decrease in activity based on the context of the message. Frequently located at critical junctions in metabolic pathways, Acetyltransferases and their regulation have become attractive therapeutic targets to treat everything from insomnia to cancer.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Acetyltransferase Positive Control 1 x 200µl Acetyltransferase Reaction Buffer Concentrate 1 x 100µl Black Microtiter Plate 1 unit Foil Plate Sealer 3 units Transferase Assay Buffer Concentrate 1 x 15ml Transferase Detection Solution Concentrate 1 x 150µl
Sequence similaritiesBelongs to the thiolase family.
- Information by UniProt
- ACAT 2
- Acetoacetyl CoA thiolase cytosolic
This product has been referenced in:
- Counihan JL et al. Chemoproteomic Profiling of Acetanilide Herbicides Reveals Their Role in Inhibiting Fatty Acid Oxidation. ACS Chem Biol 12:635-642 (2017). Read more (PubMed: 28094496) »
- Ford B et al. Mapping Proteome-wide Targets of Glyphosate in Mice. Cell Chem Biol 24:133-140 (2017). Read more (PubMed: 28132892) »