Overview

  • Product name
    Acetyltransferase Activity Assay Kit (Fluorometric)
  • Detection method
    Fluorescent
  • Sample type
    Purified protein, Inhibitor compounds
  • Product overview

    Acetyltransferase Activity Assay Kit (Fluorometric) (ab204536) is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.


    This assay is a complete kit for the screening of candidate compounds that can alter normal acetyltransferase activity. For use with purified in vitro samples.

  • Notes

    Acetylation is an important covalent molecular modification. Originally identified as the method by which certain bacteria were able to deactivate anti-microbial compounds, acetylation is now also known as an important partitioning and signaling modification.

    Acetyltransferases are enzymes that covalently transfer an acetyl group from a donor molecule (Acetyl CoA) to an acceptor. Acetyl CoA serves as a universal donor while the acceptor varies with the acetyltransferase. Acceptors include histones, kinases, transcription factors, receptors, neurotransmitter precursors like choline and serotonin, and anti-microbial agents like chloramphenicol and fluoroquinones. Acetylation can signal an increase or decrease in activity based on the context of the message. Frequently located at critical junctions in metabolic pathways, Acetyltransferases and their regulation have become attractive therapeutic targets to treat everything from insomnia to cancer.

  • Platform
    Microplate

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    Acetyltransferase Positive Control 1 x 200µl
    Acetyltransferase Reaction Buffer Concentrate 1 x 100µl
    Black Microtiter Plate 1 unit
    Foil Plate Sealer 3 units
    Transferase Assay Buffer Concentrate 1 x 15ml
    Transferase Detection Solution Concentrate 1 x 150µl
  • Research areas
  • Sequence similarities
    Belongs to the thiolase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • ACAT 2
    • Acat2
    • Acetoacetyl CoA thiolase cytosolic
    • Acetoacetyl Coenzyme A thiolase
    • Acetyl CoA acetyltransferase cytosolic
    • Acetyl CoA acetyltransferase cytosolic variant
    • Acetyl CoA transferase like protein
    • Acetyl Coenzyme A acetyltransferase 2
    • Acetyl-CoA acetyltransferase
    • Acetyl-CoA transferase-like protein
    • ACTL
    • cytosolic
    • Cytosolic acetoacetyl CoA thiolase
    • Cytosolic acetoacetyl-CoA thiolase
    • THIC_HUMAN
    see all

Images

Protocols

References

This product has been referenced in:
See all 2 Publications for this product

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