Product nameAnti-Acidic Cytokeratin antibody [AE-1]
See all Acidic Cytokeratin primary antibodies
DescriptionMouse monoclonal [AE-1] to Acidic Cytokeratin
Specificityab187381 recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily.
Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Monkey, Turtle
Recombinant full length protein corresponding to Human Acidic Cytokeratin. P13645 (CK10); P02533 (CK14); P19012 (CK15); P08789 (CK16); P08727 (CK19)
Database link: P13645
- Skin, Squamous cell carcinoma (SCC)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
- Recombinant Human Cytokeratin 16 / K16 protein (ab132964)
- Recombinant Human Cytokeratin 19 protein (denatured) (ab140055)
- Recombinant Human Cytokeratin 15 protein (ab158804)
- Recombinant Human Cytokeratin 16 / K16 protein (denatured) (ab177600)
- Recombinant Human Cytokeratin 14 protein (ab73637)
- Recombinant Human Cytokeratin 19 protein (ab73639)
Our Abpromise guarantee covers the use of ab187381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.|
|Flow Cyt||Use 0.5-1µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceCytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin.
- CK10 antibody
- Cytokeratin 10 antibody
- K10 antibody
ab187381 has not yet been referenced specifically in any publications.