Recombinant
RabMAb

Anti-Aconitase 2 antibody [EPR8283(B)] (HRP) (ab198555)

Overview

  • Product name
    Anti-Aconitase 2 antibody [EPR8283(B)] (HRP)
    See all Aconitase 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8283(B)] to Aconitase 2 (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Aconitase 2 aa 500-600. The exact sequence is proprietary.

  • Positive control
    • WB: HeLa, K562, Jurkat and HEK293 whole cell lysates. IHC-P: normal human colon tissue sections.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • Pathway
    Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
  • Sequence similarities
    Belongs to the aconitase/IPM isomerase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACO 2 antibody
    • ACO2 antibody
    • ACON_HUMAN antibody
    • aconitase 2 antibody
    • Aconitase 2 mitochondrial antibody
    • Aconitase antibody
    • Aconitase2 antibody
    • Aconitate hydratase antibody
    • Aconitate hydratase mitochondrial antibody
    • ACONM antibody
    • Citrate hydro lyase antibody
    • Citrate hydro-lyase antibody
    • ICRD antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (HRP) (ab198555) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 20 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198555 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Aconitase 2 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198555 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab198555 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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