Anti-Adenosine A1 Receptor antibody (ab3460)
Key features and details
- Rabbit polyclonal to Adenosine A1 Receptor
- Suitable for: WB, ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Adenosine A1 Receptor antibody
See all Adenosine A1 Receptor primary antibodies -
Description
Rabbit polyclonal to Adenosine A1 Receptor -
Host species
Rabbit -
Specificity
Detects Adenosine Receptor A1. This antibody does not detect other AR subtypes. -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Rat Adenosine A1 Receptor aa 309-326.
Sequence:CQPKPPIDEDLPEEKAED
(Peptide available asab5893) -
Positive control
- WB: PC-12, SH-SY5Y, U-87MG, A549, K-562 whole cell lysates. ICC: HeLa cells IHC: rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Predicted molecular weight: 37 kDa.Can be blocked with Adenosine A1 Receptor peptide (ab5893).
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ICC |
Use a concentration of 2 µg/ml.
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IHC-P |
1/50 - 1/200.
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Notes |
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WB
1/500. Predicted molecular weight: 37 kDa.Can be blocked with Adenosine A1 Receptor peptide (ab5893). |
ICC
Use a concentration of 2 µg/ml. |
IHC-P
1/50 - 1/200. |
Target
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Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 282133 Cow
- Entrez Gene: 134 Human
- Entrez Gene: 29290 Rat
- Omim: 102775 Human
- SwissProt: P28190 Cow
- SwissProt: P30542 Human
- SwissProt: P25099 Rat
- Unigene: 77867 Human
see all -
Alternative names
- A1AR antibody
- A1R antibody
- AA1R antibody
see all
Images
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Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized HeLa cells staining adenosine A1 receptor with ab3460 at 2 µg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin is stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control contains no primary anibody.
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All lanes : Anti-Adenosine A1 Receptor antibody (ab3460) at 1/250 dilution
Lane 1 : PC-12 whole cell extract
Lane 2 : SH-SY5Y whole cell extract
Lane 3 : U-87 MG whole cell extract
Lane 4 : A549 whole cell extract
Lane 5 : K-562 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa -
ab3460 labelling Adenosine A1 receptor in the cytoplasm of Rat brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Datasheets and documents
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Datasheet download
References (4)
ab3460 has been referenced in 4 publications.
- Rivera-Oliver M et al. Adenosine A1-Dopamine D1 Receptor Heteromers Control the Excitability of the Spinal Motoneuron. Mol Neurobiol 56:797-811 (2019). PubMed: 29797183
- Stettler O et al. Engrailed homeoprotein recruits the adenosine A1 receptor to potentiate ephrin A5 function in retinal growth cones. Development 139:215-24 (2012). PubMed: 22147955
- Datino T et al. Mechanisms by which adenosine restores conduction in dormant canine pulmonary veins. Circulation 121:963-72 (2010). WB ; Dog . PubMed: 20159830
- Kempson SA et al. Acute inhibition of the betaine transporter by ATP and adenosine in renal MDCK cells. Am J Physiol Renal Physiol 295:F108-17 (2008). WB ; Dog . PubMed: 18448594