Anti-Aggrecan ARGxx antibody [BC-3] (ab3773)
Key features and details
- Mouse monoclonal [BC-3] to Aggrecan ARGxx
- Suitable for: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WB
- Reacts with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Aggrecan ARGxx antibody [BC-3] -
Description
Mouse monoclonal [BC-3] to Aggrecan ARGxx -
Host species
Mouse -
Specificity
Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Samples need to be deglycosylated for epitope recognition (see notes). -
Tested applications
Suitable for: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
Does not react with: Mouse -
Immunogen
Synthetic peptide corresponding to Aggrecan ARGxx.
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General notes
Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 3.16% Tris HCl -
Concentration information loading...
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Purity
Tissue culture supernatant -
Primary antibody notes
Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease. -
Clonality
Monoclonal -
Clone number
BC-3 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24312401
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ELISA | (1) |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration. PubMed: 19932675
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IHC-Fr |
Use at an assay dependent concentration.
This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections. |
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IHC-P |
Use at an assay dependent concentration.
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections. |
|
WB |
Use at an assay dependent concentration.
Detects a variety of epitopes between 50 and 250 kDa. |
Notes |
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ICC/IF
Use at an assay dependent concentration. PubMed: 24312401 |
ELISA
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 19932675 |
IHC-Fr
Use at an assay dependent concentration. This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections. |
IHC-P
Use at an assay dependent concentration. This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections. |
WB
Use at an assay dependent concentration. Detects a variety of epitopes between 50 and 250 kDa. |
Target
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Relevance
Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 280985 Cow
- Entrez Gene: 403828 Dog
- Entrez Gene: 176 Human
- Entrez Gene: 397255 Pig
- Entrez Gene: 58968 Rat
- Omim: 155760 Human
- SwissProt: P13608 Cow
- SwissProt: Q28343 Dog
see all -
Alternative names
- AGC1 antibody
- Aggrecan 1 antibody
- Aggrecan core protein antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab3773 has been referenced in 70 publications.
- Li XC et al. M2 macrophage-conditioned medium inhibits intervertebral disc degeneration in a tumor necrosis factor-α-rich environment. J Orthop Res 40:2488-2501 (2022). PubMed: 35170802
- Boneski PK et al. Abcc6 Null Mice-a Model for Mineralization Disorder PXE Shows Vertebral Osteopenia Without Enhanced Intervertebral Disc Calcification With Aging. Front Cell Dev Biol 10:823249 (2022). PubMed: 35186933
- Du X et al. Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression. Cell Prolif 55:e13205 (2022). PubMed: 35187741
- Aidos L et al. Meniscus Matrix Structural and Biomechanical Evaluation: Age-Dependent Properties in a Swine Model. Bioengineering (Basel) 9:N/A (2022). PubMed: 35324808
- Miyazaki K et al. Protective Effects of Growth Differentiation Factor-6 on the Intervertebral Disc: An In Vitro and In Vivo Study. Cells 11:N/A (2022). PubMed: 35406739