Anti-Ago1 antibody (ab5070)
Key features and details
- Rabbit polyclonal to Ago1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Drosophila melanogaster
- Isotype: IgG
Overview
-
Product name
Anti-Ago1 antibody
See all Ago1 primary antibodies -
Description
Rabbit polyclonal to Ago1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Drosophila melanogaster -
Immunogen
Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab23538) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | (2) |
Use at an assay dependent concentration. PubMed: 18463631
|
IHC-P |
Use at an assay dependent concentration. PubMed: 18528333
|
|
WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
|
Notes |
---|
IP
Use at an assay dependent concentration. PubMed: 18463631 |
IHC-P
Use at an assay dependent concentration. PubMed: 18528333 |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. |
Target
-
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
-
Database links
- Entrez Gene: 36544 Drosophila melanogaster
- Entrez Gene: 236511 Mouse
- SwissProt: Q8CJG1 Mouse
- Unigene: 30800 Mouse
-
Alternative names
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
Images
-
Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Anti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (49)
ab5070 has been referenced in 49 publications.
- Lim LX et al. The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis in D. melanogaster. Front Mol Biosci 9:818302 (2022). PubMed: 35425810
- Liu P et al. Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3p. Front Cell Dev Biol 10:879877 (2022). PubMed: 35557954
- Gao Y et al. Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells. STAR Protoc 3:101596 (2022). PubMed: 35928005
- Rozen-Gagnon K et al. A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Sci Rep 11:736 (2021). PubMed: 33436886
- Kingston ER & Bartel DP Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation. RNA 27:710-724 (2021). PubMed: 33853897