Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112)

Overview

  • Product name
    Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Serum, Cell Lysate, Tissue Lysate
  • Assay type
    Quantitative
  • Sensitivity
    0.001 mU/well
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112) provides a convenient tool for sensitive detection of Aldo-Keto Reductase (AKR) activity in animal tissue and cells, as well as biological fluids such as serum. The assay is based on the ability of AKR to reduce a general substrate and convert NADP+ to NADPH, which reacts with the AKR probe to generates color that can be detected at OD 450 nm. The intensity of the color is proportional to the activity of AKR in the sample. 


    This assay can detect as low as 1 µU of AKR activity in samples. It has been validated with AKR1B10, AKR1C1 and AKR1C3.

  • Notes

    Aldo-keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases which contains more than 190 members and are present in nearly all phyla. They are mainly monomeric soluble proteins (34 – 37 kD) and their common is to oxidize/reduce aldehydes or ketones to their corresponding primary or secondary alcohols. Due to their broad substrate specificity, AKRs play roles in intermediary metabolism, biosynthesis and detoxification.

    In humans, AKRs are not only involved in carbonyl metabolism, but they also regulate signaling through nuclear receptors which leads to chemical carcinogenesis. Many studies demonstrate increasing expression of AKRs in cancers such as lung, liver and colon cancers.

  • Platform
    Microplate

Properties

Images

  • Typical NADPH standard curve.

  • Kinetic curves showing AKR activity detection in increase amounts of positive control included in the kit (μL/assay).

  • AKR specific activity in human tissue lysates and cell line lysates. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve.

Protocols

References

ab211112 has not yet been referenced specifically in any publications.

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